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For the GSSCys assays, either GSH was varied between 50 M and 2.0 mM at fixed GSSCys concentrations (25, 50, 100 and 150 M) or GSSCys was varied between 25 and 200 M at fixed concentrations of GSH (0.05, 0.1, 0.2, 0.3 and 1.0 mM for ScGrx7 or 0.3, 0.5, 1.0 and 2.0 mM for PfGrx)
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Therefore, CK activity can be used as a surrogate marker for the leakage of these cellular metabolites, which confirms membrane rupture
Presented for research literature review only Morinobu S et al
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